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The 6His tag was removed by thrombin digestion overnight at 4 C, and ApoA1 was further purified by anion exchange chromatography (HiTrap Q, Cytiva) followed by size-exclusion chromatography (Superdex 200 Increase 10/300 GL, Cytiva)

Because cysteine is relatively scarce in the standard diet, its availability often acts as the bottleneck for glutathione production

However, B12 shots can lead to side effects including: - Headache - Dizziness - Diarrhea - Skin rash and itching - Nausea and vomiting - Fatigue - Swelling and tingling sensation Additionally, excess vitamin B12 can also result in rare but severe allergic reactions also known as anaphylaxis

High Dosage Injections typically provide a much higher dose of Vitamin B12 compared to oral supplements