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Antioxidants fight harmful particles in the body known as free radicals, which damage cells and tamper with DNA
TUNEL assay On experimental day P38, pancreas samples were fixed in 2% w/v paraformaldehyde for 2 h and processed for TUNEL assay, which was performed using the One Step TUNEL Apoptosis Assay Kit (Beyotime, Shanghai, China) according to the manufacturers instructions
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These observations in RNA-seq data were further validated by qRT-PCR for selected Wnt/-catenin pathway-related genes including AMER3 , NRARP , CCND1 , LRG1 , and LEF1 in hDP cells exposed with various doses of NTAPP (3.6 kV, 4.2 kV, and 5.0 kV) (Fig
