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Culturing was performed in RPMI 1640 medium enriched with 10% fetal bovine serum, maintained at 37C in a humidified incubator with an atmosphere of 5% CO 2 and 95% air

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(95) have elegantly demonstrated that at least half of the extracellularly available glucose in the brain is accumulated by neurons and that lactate is metabolized by both neurons and astrocytes

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