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Initial inhibitor screens were conducted in 200 l volumes in 96 well plates with 50 M inhibitor, 100 M NADPH, and 5 nM TGR in 0.1 M potassium phosphate (pH 7.4), 10 mM EDTA with a preincubation step of 15 min
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Unexplained Weight Gain or Loss: Significant changes in weight, either gain or loss, can accompany delayed menstruation
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