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Oktay et al., 2024)
Alternatively, glutathione can be quantified after reduction of GSSG in the sample with dithiotreitol (DTT) and subsequent derivatization of GSH with mbrB or mchlB via a reverse-phase high-performance liquid chromatography (HPLC) system equipped with a fluorescent or UV detector, or spectrophotometrically after a GR-mediated glutathione recycling assay (total glutathione) from cell organelles after isolation or fractionation
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This delay prolongs nutrient absorption and stabilizes post-meal glucose responses, reducing spikes that can contribute to fat accumulation and energy crashes
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