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(b) impaired MFO and MGO

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The bound protein is washed under conditions that do not disrupt the specific interaction, but that can disrupt any non-specific interactions between contaminating proteins and the stationary phase

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Cells were fixed 6, 24, or 49 h post-infection in 4 % formaldehyde, washed in PBS, permeabilised in 0.25 % Triton X-100, and blocked in 4 % BSA

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& Jimenez, A

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