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The resulting PCR products representing partial cDNAs of differentially expressed nucleotide sequences are then cloned (i.e., ligated) into an appropriate vector (e.g., a commercially available TA cloning vector, such as pGEM from Promega) and, optionally, transformed into competent bacteria for selection and screening

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When youre hiking, camping, skiing, fishing or whatever you do outdoors, you might as well charge your stuff while youre at it

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