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A 2002 systematic review published in the American Journal of Medicine has found that milk thistle has therapeutic benefits for liver disease ( 14 )

To extract hydrogen peroxide (H 2 O 2 ) from leaf tissues, 50 mg of the tissue was homogenized in 3 mL of phosphate buffer (50 mM, pH 6.5)

10.3389/fnbeh.2021.675206 Summary Keywords microbiotagutbrain axis, neurodegenerative diseases, microplastics, gut dysbiosis, neuropsychiatric disorders, microbiome-based interventions, neuroinflammation Citation Yassin LK, Skrabulyte-Barbulescu J, Alshamsi SH, Saeed S, Alkuwaiti SH, Almazrouei S, Alnuaimi A, BaniYas S, Aldhaheri D, Alderei M, Shehab S and Hamad MIK (2025) The microbiotagutbrain axis in mental and neurodegenerative disorders: opportunities for prevention and intervention