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Sahu A, et al

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I recommend: at least 2,000 mcg (g) cyanocobalamin once each week, ideally as a chewable, sublingual, or liquid supplement taken on an empty stomach or at least 50 mcg daily of supplemental cyanocobalamin or a serving of B12-fortified foods three times a day, each containing at least 190 percent of the Daily Value listed on the nutrition facts label Those over 65 years of age should take at least 1,000 mcg (g) cyanocobalamin every day

A microtome was used to cut the embedded samples into 60 nm ultrathin sections, which were then stained with 2% uranyl acetate at room temperature in the dark for 20 min and lead citrate at room temperature for 10 min