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We hypothesize that conformer 1 is required for G protein activation, and that adoption of this receptor conformation is favored under conditions used to form a stable complex that can be imaged (inclusion of dominant negative G protein and nanobody 35, apyrase treatment)

On the food side: bitter foods like arugula, radicchio, dandelion greens trigger bitter taste receptors in the gut that stimulate GLP-1, and turmeric and ginger also have emerging evidence

The observed inconsistencies across studies likely result from variations in sample sizes, ethnic backgrounds, and age groups

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