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Hence, depending upon the extent of glutaric acid deficiency, there may be an over production of serotonin (mod deficiency), whereas in larger deficiency there is an underproduction of serotonin
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TMA-containing compound assay Trimethylamine-containing compounds were extracted from 50 l of plasma, 50 l of whole liver homogenate or from 100 l of whole brain homogenate (without the cerebellum and olfactory bulbs) by adding 10 volumes of ACN and spiking the samples with 5 l of IS mix containing 12 M d9-TMAO (CIL), 649 nM d9-TMA (CDN Isotopes), 6 M d3-L-carnitine (CIL), 329 nM d9-crotonobetaine (see An LTQ Orbitrap MS interfaced to an Agilent 1200 LC was used for LCMS/MS analysis
