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Maraganore et al., 1999), which encode the ubiquitin E3 ligase parkin and deubiquitinating enzyme UCHL1, respectively
Since D2HG and L2HG are indistinguishable in standard LC-MS, to determine which 2HG enantiomer(s) is affected, we adapted a chiral N-(p-toluenesulfonyl)-L-phenylalanyl chloride (TSPC) derivation followed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis 24,40
Detection methods can identify the peptide and its metabolites in both blood and urine
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