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Genomic DNA of fecal samples was extracted using the cetyltrimethylammonium bromide method and was used for 16S rRNA sequencing
Surgery success rates are generally good for pain relief (70 to 90% improvement in leg pain symptoms), but come with risks: infection, nerve damage, disc reherniation (5 to 15% recurrence rate after microdiscectomy), and adjacent segment disease with fusion (20 to 30% develop problems within 10 years)
doi: 10.1016/S0741-8329(02)00229-X 69
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