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This can cause a variety of problems, such as muscle and joint pain, weakness, and vulnerability to infections and injuries

Generation of cell lines To generate the HeLa Lap2b:RFP/Lifeact:GFP cell line, HeLa cells stably expressing Lap2b fused to RFP (a gift from Daniel Gerlich, Institute of Molecular Biotechnology, Vienna, Austria) (Steigemann et al, 2009) were transfected with the pEGFP-C1/Lifeact-EGFP plasmid (#58470, Addgene) coding for Lifeact peptide fused to EGFP inside the pEGFP-C1 vector, conferring resistance to neomycin (Invitrogen)

Dermatol Ther 35(7):e15544

TB-500 primarily promotes actin polymerization and cell migration, while BPC-157 appears to act more directly on fibroblast proliferation and collagen deposition