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The disulfide bond between Pt GSTF8 residue C13 and GSH was reduced on-column with 20 column volumes of rPPS buffer (20 mM potassium phosphate, 200 mM NaCl, 10 mM DTT, pH 7.4), at a flow rate of 5 ml min 1 and the column was then washed with 40 column volumes of PPS buffer at a flow rate of 5 ml min 1 to remove free GSH

Because hydrophobic bile acids are harmful to hepatocytes and cholangiocytes, obstruction of bile flow causes retention of bile acids in the liver, resulting in liver damage ( Individuals with prolonged severe intrahepatic cholestasis (PSIC) have a higher mortality risk (44%) than patients without PSIC (20%) after orthotopic liver transplantation ( in vivo studies

500 l of chloroform was added to the cells and the suspension was vortexed for 1 hour at 4 C

GHKCU Vial (Copper Tripeptide1) is a naturally occurring peptide complex formed when the tripeptide sequence GlyHisLys binds copper (II) ions