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Primary cells and immortalized cell lines are generally cultured under atmospheric oxygen levels, which are higher than the physiological oxygen content in vivo , leading to a pro-oxidative environment in vitro (Sies et al., 2022)
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doi: 10.1016/j.cmet.2020.06.010 139 KernanK
doi: 10.1093/hmg/ddu243 255 ShengY.ChattopadhyayM.WhiteleggeJ.ValentineJ
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