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GSH synthesis using the whole-cell biocatalyst was performed in 0.2 M phosphate buffer (pH 7.0) containing 40 mM l-glutamate, 20 mM l-cysteine, 40 mM glycine, 20 mM MgCl 2 and 20 mM ATP
Lands et al., 1999
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After 2 min, 0.1 ml samples were diluted to a final volume of 1 ml containing 1 mM GSH, 1 mM CDNB in 0.1 M potassium phosphate buffer, pH 6.5, according to the standard procedure of Habig et al
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