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Freeze-thaw cycling can disrupt the copper coordination sphere in addition to degrading the peptide backbones, making single-use aliquots preferable to repeated access of one stock
The most critical factor for preservation is refrigerating the vial *after* it has been mixed
When the nerve becomes inflamed, symptoms can include: Shooting or radiating pain down the leg Burning or electric sensations Numbness or tingling Weakness in the leg or foot Pain that worsens with sitting What is often overlooked is that irritated nerves become inflamed at a microscopic level
Anjmun Sharma , at Blooming Wellness & Weightloss Clinic to determine if B12 injections are right for you
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