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Sodium-proton exchanger activity was determined in cells loaded with the fluorescent cytosolic pH indicator 2'7'-biscarboxyethyl-5,6-carboxyfluorescein acetoxymethylester (BCECF) after pretreatment with 250 nM of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate for 10 min

Additionally, radioimmunoprecipitation assays (RIPA) and Western blot tests were conducted, and the corpus cavernosum of the penis was histopathologically examined

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