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Current research done on the potential beneficial effects of metformin has extended to treating diabetic nephropathy (Han et al., 2021), metabolic syndrome (Ladeiras-Lopes et al., 2015), and polycystic ovary syndrome (Peng et al., 2023)

The protein samples were digested with trypsin (trypsin: protein = 1:20, w/w) at 37 C

Beneficial bacteria produce anti-inflammatory compounds and strengthen the gut barrier, which reduces systemic inflammation

The change in body weight was evaluated by the researchers, who then used mediation analysis to distinguish between the direct and indirect effects of weight reduction (mediated by GI AEs)