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Mechanisms and treatment of late-life depression

Using a 110 l multichannel pipette, 5 l of NADPH solution in TBS or vehicle only was then added followed by 5 l of DTUN solution (12 mM) in ethanol to give a final volume of 300 l (final concentration of 1 mM egg-PC, 1 M STY-BODIPY, 4 M inhibitor, 16 nM mFSP1, 320 nM FAD, 0.2 mM DTUN and NADPH varying between 4 and 64 M)