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With this notion, the study of these molecular transducers has been approached by some authors in recent years from a top-down point of view, whereby sub-type specific, allosteric positive or negative modulators (PAM, NAMs), as well as direct agonists, are utilized as tools for pathway dissection and analysis ( The expression of different GPCRs to restricted anatomical regions, such as the enteroendocrine cell system, is a finely tuned system that evolved in metazoan

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