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A study of humans with a genetic mutation for CYP2R1, or those done using CYP2R1 knockdown mouse models, demonstrate low circulating levels of 25(OH)VD 3 24
Nevertheless, the most likely conclusion up to date would be that it was indeed taken up as sorbitol was added to the medium and might easily been taken up as it is water-soluble
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A reverse primer specific to the orf1a sequence of SARS-CoV-2 (5-CGTGCCTACAGTACTCAGAATC-3) was annealed to the extracted RNA and reverse transcribed into cDNA using SuperScript III Reverse Transcriptase along with RNase Out
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