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Blood samples were collected according to a standardised operating procedure using 0.109 M buffered sodium citrate for the CTC preparation, BD Vacutainer tubes containing K 2 EDTA for plasma samples and BD Vacutainer tubes containing clot activator and gel separation for serum samples

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Customized protocols may combine topical and injectable GHK-Cu delivery methods with complementary treatments such as platelet-rich plasma , microneedling , prescription medication( such as Minoxidil) , and nutritional optimization