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High-dose oral formulations (5,000+ mcg) can work if taken sublingually and held under the tongue for absorption, but consistency is critical
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Cells were transfected at 80% confluency using Lipofectamine 2000 (Thermofisher) Briefly, 2 g pcDNA3.2- aptf2::V5 was diluted in 200 l Opti-MEM (Life Technologies) and 8 l Lipofectamine was diluted in 200 l Opti-MEM
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