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After rinsing the sections, the following day with 1X PBST (containing 0.1% Tween 20), they were incubated for 1 h at room temperature (avoided direct exposure to light) with the respective secondary antibodies, Alexa Flour 488 and Alexa Flour 555 goat anti-rabbit and Alexa Fluor 647 goat anti-mouse

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Originally investigated for its potential to combat obesity, metabolic syndrome, and age-related decline, this NNMT (nicotinamide N-methyltransferase) inhibitor has shown promise in boosting fat metabolism, preserving lean muscle mass, and increasing energy efficiency

When these molecules outweigh the body's antioxidant defenses, a state called oxidative stress occurs