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Measurement of TMA, d 9 -TMA, TMAO, d 9 -TMAO, BB, d 9 -BB, carnitine, and d 9 -carnitine levels in mice by LC-MS For sample preparation, 50 L of mouse serum was added to 450 L of methanol containing isotopically labeled internal standards (d 3 -carnitine, d 9 -TMAO, and 13 C 3 -TMAO)
Lippmann ES, Azarin SM, Kay JE, Nessler RA, Wilson HK, Al-Ahmad A, et al
Local ice application typically lasts 15-20 minutes per session, limited to prevent tissue damage from prolonged cold exposure
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