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The second step after filtering was performed with 0.23 M Pt GSTF8 and varying dilutions of the first step reaction in buffer to cover substrate concentrations from 389 M to 6.5 M
We exposed cell lines that had either Keap1, Nrf2 or Srxn1 tagged with green fluorescent protein (GFP) to a concentration range of DEM and monitored the response of these Nrf2 pathway components using high throughput confocal imaging (Fig
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