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To investigate the depletion of O 2 within the cells, cancer cells in a 1% O 2 atmosphere were treated with Ru(II)-OH, NP2, or NP4, and the O 2 concentration was assessed using the hypoxia probe pimonidazole hydrochloride

This method is often used in clinical settings and may be recommended for individuals with specific health concerns

The studys authors noted that increases in GH and IGF-I levels produced by CJC-1295 DAC were dose-dependent, meaning that in the context of that particular study, higher doses of CJC-1295 produced greater rises in GH and IGF-1 levels than lower doses