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Particularly, using Asiatic acid in interstitial cystitis has proven to be successful in rats model of cystitis induced by cyclophosphamide, this resulted in the decrease of bladder pressure, detrusor overactivity the normalization of biomarker levels and reduction of bladder edema and thickness of urothelium

Subsequently, the cell monolayer was scratched using a 200 L pipette and then washed twice with PBS

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