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DNA dot-blots for 5-mC and 5-hmC revealed the patterns created by the events of the epigenetic modification enzymes

Plasma levels of total GSH, free GSH, GSSG, and the ratio of free GSH to GSSG were then independently analyzed to produce 16 measures per animal

To assess oxidative stress and antioxidant capacity in testis, levels of malondialdehyde (MDA), glutathione (GSH), folate and susceptibility index (SI) of pro-oxidative damage, mRNA expression of Nrf2, NFB-p65, IL-6, IL-10 and TNF-, in addition to superoxide-dismutase (SOD), catalase and glutathione-peroxidase (GPx) activities were measured

The first signs are often noticeable from weeks 2 to 4: a little more energy and less fatigue