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Real-time quantitative PCR (RT-qPCR) was performed in triplicate on a CFX96 system (Bio-Rad, Hercules, CA, USA) using SYBR Green Mix (Toyobo, Osaka, Japan)
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Toxicology assessments in rats found no organ toxicity at doses up to 10 mg/kg (approximately 1000x the effective dose), no mutagenicity in Ames testing, and no teratogenic effects
Cravings are at a minimum as well
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