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After cryoprotection with 30% sucrose in 0.1 M PBS brains were sectioned coronally at 30 m thickness

Debido a que la sal aadida a menudo se encuentra en alimentos abiertamente calricos y ricos en grasa, si ves cifras altas de sodio o sal en una etiqueta nutricional debera hacerte reflexionar si te preocupa el malestar estomacal

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Plasmid construction To generate CRISPR-Cas9 plasmids for gene manipulation in AG-haESCs, specific sgRNAs were synthesized, annealed, and inserted into the BpiI (Thermo Fisher Scientific, Waltham, MA, USA)-linearized pX330-mCherry plasmid (Cat# 98750