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Structurally, three regions are distinguished in Pit-1: (1) a DNA-binding zone located at the C-terminal end, in which two domains can be differentiated, a homeodomain (POU HD ) of 60 amino acids found throughout the family of transcription factors with POU domains, and a specific POU domain (POU s ) of 75 amino acids located at the N-terminus of the POU HD domain

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Additionally the study found that during the process of cell division there were fewer spindle defects and decreased abnormalities of the cortical granules when evaluated at 12 and 24 hours