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The samples were rotated at room temperature for 3 hours before washing with the 2 nd wash buffer (50 mM TEAB, 150 mM NaCl, 0.1% NP-40) 3 times, 3 times with PBS and 3 times with water
While sermorelin contains just the first 29 amino acids of the GHRH protein, its mechanism of action is identical
2) Cellular Growth in Human Myotubes In human cell models 4 , IGF-1 directly drives hypertrophy markers (greater nuclei per myotube, higher fusion index, elevated contractile protein content), reinforcing its role in the cellular foundation of strength and size gains
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