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Tsukagoshi et al., 2010)

Tirzepatide Reconstitution Math Tirzepatide - dose per draw at common vial sizes Vial Size 5 mg BAC Water 1.0 mL Concentration 5 mg/mL 2.5 mg 0.50 mL (50 units) 5 mg 1.00 mL (100 units) 10 mg N/A 15 mg N/A Vial Size 10 mg BAC Water 2.0 mL Concentration 5 mg/mL 2.5 mg 0.50 mL (50 units) 5 mg 1.00 mL (100 units) 10 mg 2.00 mL (200 units*) 15 mg 3.00 mL (300 units*) Vial Size 10 mg BAC Water 1.0 mL Concentration 10 mg/mL 2.5 mg 0.25 mL (25 units) 5 mg 0.50 mL (50 units) 10 mg 1.00 mL (100 units) 15 mg 1.50 mL (150 units*) Vial Size 30 mg BAC Water 3.0 mL Concentration 10 mg/mL 2.5 mg 0.25 mL (25 units) 5 mg 0.50 mL (50 units) 10 mg 1.00 mL (100 units) 15 mg 1.50 mL (150 units*) * Volumes above 100 units exceed a standard 1 mL insulin syringe

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body mass index (BMI) of 21.033.0 kg/m 2 , sedentary lifestyle (defined by Stanford questionnaire, Additional file 1: Table S1), maintain current dietary habits and activity levels, if taking supplements agree to maintain dosing regimen for at least one month prior to baseline and during the study, agree not to start any new supplements, give voluntary, written informed consent to participate