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Extinction coefficients were measured by creating a dilution series (115 M), measuring the absorbance at max and applying a linear regression
After incubation of the blood in the presence of acetylphenylhydrazine, the GSH-level was 119 mg per cent (normal range 6094 mg per cent)
Plasmid construction To generate CRISPR-Cas9 plasmids for gene manipulation in AG-haESCs, specific sgRNAs were synthesized, annealed, and inserted into the BpiI (Thermo Fisher Scientific, Waltham, MA, USA)-linearized pX330-mCherry plasmid (Cat# 98750
The nuclear protein fraction was extracted, and the relative Dnmt and 5-mC hydroxylase Tet activities were calculated based on the ratio of the treatment group to the control group after the normalization to the amount of protein
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