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Accompanying the inward movement of GIPR ECL1 by 6.4 relative to that of GCGR (measured by C of G202 ECL1 in GIPR and G207 ECL1 in GCGR), the C terminus of peptide 20 bound by GIPR shifted toward TMD core by 8.1 (measured by C of L27 P ) and consequently pushed the extracellular tip of TM1 moving toward TM7 by 2.8 (measure by C of the residues at 1.29b)

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On the other hand, no significant differences in active GLP-1 and GIP were noted in plasma isolated immediately in the same mice by CP (Figure 2, E and F)

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