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Experiments are in compliance with the ARRIVE guidelines ( Expression and purification of recombinant oIGF-1 The recombinant oIGF-1-N-terminal-protease (NPro) fusion protein coding sequence was cloned into pD451-SR plasmid under T7 promoter and transformed into E

However, particle diffusion is not substantially enhanced, likely because the DNA fragments generated still increase the micro-viscosity within mucus pores, ultimately limiting particle mobility 197

The mechanism may be related to the decrease of forkhead-box class O (FOXO) transcription factor expression in chondrocytes, thus weakening the cell's resistance to oxidative stress [41]

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