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Briefly, i4F MEFs were seeded at a density of 3 10 5 cells per well in six-well tissue culture plates coated with gelatin and treated with doxycycline (PanReac, A2951) 1 mg ml 1 continuously to induce expression of the OSKM transcription factors in the presence of complete KSR media (15% (vol/vol) Knockout Serum Replacement (KSR, Invitrogen, 10828028) in DMEM with GlutaMax (Life Technologies, 31966047) basal media, with 1,000 U ml 1 LIF (Merck, 31966047), non-essential amino acids (Life Technologies, 11140035) and 100 M beta-mercaptoethanol (Life Technologies, 31350010) plus antibiotics (penicillinstreptomycin, Gibco, 11528876)), which was replaced every 4872 h
Although the number of injections that would be administered would very much depend on the extent of the nerve damage, they are normally administered over several weeks or months
Unlike injectable forms, their most distinctive feature is a convenient, safe, and easy-to-follow administration route that requires no professional operation and is suitable for daily use, while stably exerting metabolic regulatory effects.Produced with precise formulation technology, each tablet has a controllable dose (conventional range: 50150 mg) and can be split as needed
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