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We emphasize the importance of avoiding foods high in refined sugars and processed carbohydrates, as these can cause rapid increases in blood sugar and make weight management more challenging

In the considered dose range of 0.5 to 1 mg kg 1 , AMG 133 demonstrates a long half-life ranging from 189 to 222 h (intact AMG 133 assay) and from 231 to 284 h (total AMG 133 assay)

After washing once with Annexin-V binding buffer, cells were acquired via flow cytometry (LSRII, BD Bioscience, Heidelberg, Germany) and data were analyzed with FlowJo X (FlowJo LLC, Ashland, OR, USA)