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Additionally, the designing of different sensors has used the circularly permutated fluorescent protein (cpFP), where the original N and C termini were replaced by a short peptide linker in order to alter the pKa values and orientation of chromophore from their original counterpart 11,12
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For metabolites glutamate and glutamine, a whole spectral area representing one hydrogen was integrated, for GABA and NAA spectral area representing 2, resp
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