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The primary antibodies used included anti-ZO-1 (Abcam, ab307799, 1:5000), anti-Occludin (Abcam, ab216327, 1:5000), anti-ACSL4 (Abcam, ab155282, 1:10000), anti-TFR1 (ABclonal, A5865, 1:2000), anti-SLC7 A11 (Abcam, ab175186, 1:5000), anti-GPX4 (Abcam, ab125066, 1:5000), anti-VDAC2 (Proteintech, 116631-AP, 1:5000), anti-VDAC3 (Proteintech, 552601-AP, 1:5000), -tubulin (Proteintech, 100681-AP, 1:5000), and GAPDH (Proteintech, 104941-AP, 1:20000)
Interestingly, it is a selective stimulator of the growth hormone, which means that it does not affect other hormonal levels
Sample Collection Serum: Allow samples to clot for 1 hour at room temperature or overnight at 28C before centrifugation at 1,000g for 20 minutes at 28C
In general, these blends are discussed as different pathway-focused options rather than required combinations
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