cDNA was synthesized with the iScript cDNA Synthesis Kit (#170-8891, Biorad) and subjected to quantitative real-time PCR using SYBR Green Supermix (#172-5272, Biorad)

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In addition to bringing Vanderbilts first-ever satellite graduate campus to town, he was instrumental in partnering with Guident Corp
The choice between pen and vial is therefore above all a matter of how the reference material is stored and handled in the laboratory