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After 24 h, the transfected cells were seeded onto 384-well microtiter plates (3000 cells per well) in HBSS supplemented with 5 mM HEPES, 0.1% (w/v) bovine serum albumin (BSA) and 0.5 mM 3-isobutyl-1- methylxanthine, and stimulated with increasing concentrations of GLP-2 for 30 min at RT

Metformin reduces hepatic glucose production (the livers overnight glucose release) and modestly improves insulin sensitivity at the muscle