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Wada T, Anker SD, Liu Z, et al

Junwei S, Vakoc CR

For example, transforming growth factor beta 1 (TGF1), a multifunctional cytokine, can alter the tumor microenvironment when dysregulated, promoting angiogenesis and immune suppression, thus influencing tumor progression

After incubation (10 min, 39C), the plates were centrifuged (200 x g, 5 min, 20C, Universal 320, Hettich, Tuttlingen, Germany) and the medium was replaced with pre-warmed XF Seahorse medium supplemented with glucose, sodium pyruvate and glutamine (all purchased from Agilent, Santa Clara, California, USA)

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