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RNA extraction Platypus and echidna tissues were obtained from adult animals in accordance with ethics guidelines (approved by Adelaide University Animal Ethics Committee permit AEC S-49200 to F.G)
Furthermore, Protein Atlas indicates that GLP-1 receptor protein (but not mRNA) is present in skeletal muscle, whilst conversely GLP-1 receptor mRNA (but not protein) is detected in adipose tissue
Tailor et al
Individual donor data sets were filtered using the following quality control metrics: we excluded genes detected in fewer than 5 cells (per subject), we excluded cells with fewer than 500 UMIs, and we excluded cells with higher than 5% of mitochondrial genes
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