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After washed twice with cold PBS, the cells were suspended with 200 l of PBS, and supplemented with 10 l of RNase A stock (#10405ES03, Yeasen) solution and incubated 60 min at room temperature

All lines of evidence indicate that fast and slow TGR inhibitors bind different conformational states present in the TGR enzymatic cycle induced by both NADPH-dependent reduction and the concomitant destabilization of the polypeptide chain, as shown by TSA

Journal of Analytical Oncology, 3 (2), 79-87