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Plasmids of wildtype and mutants of ARE motifs were transfected to K562 (#CRL-3344, ATCC), an erythroleukemia cell, along with pDsRed1-N1 (PT3405, Clontech), which is used as a transfection efficacy control

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The patient attempts to go to bed about hour earlier each night and the timing of application of melatonin and light is changed to maintain the initial relationship