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Impairment of these redox currency systems compromises both antioxidant defense and mitochondrial bioenergetic stability [83]

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Peptides were desalted using StageTips (Thermo Fisher Scientific), dissolved in solvent A (0.1% (v/v) FA), and resolved on a 75 m 50 cm, 2 m particle size, EASY-Spray PepMap RSLC C18 column (Thermo Fisher Scientific) heated to 45 C, and coupled to a 75 m 2 cm, 3 m particle size, Acclaim PepMap 100 pre-column (Thermo Fisher Scientific) run on an EASY-nLC 1000 UHPLC (Thermo Fisher Scientific)